LYMPHOID NEOPLASIA Brief report Simple genetic diagnosis of hairy cell leukemia by sensitive detection of the BRAF-V600E mutation
نویسندگان
چکیده
1Institute of Hematology, University of Perugia, Perugia, Italy; 2Hematology, University of Siena and Azienda Ospedaliera Universitaria Senese, Siena, Italy; 3Department of Clinical and Experimental Medicine, Hematology and Clinical Immunology Section, University of Padua, Padua, Italy; 4Department of Medicine, Hematology Section, University of Verona, Verona, Italy; 5Department of Pathology, Center for Experimental Research and Medical Studies, University of Torino, Turin, Italy; and 6Division of Hematology, Department of Cellular Biotechnologies and Hematology, Sapienza University, Rome, Italy
منابع مشابه
بررسی ارزش تشخیصی ردیابی جهشBRAF V600Eدربیماران ایرانی مبتلابه لوسمی سلول مویی
Abstract Background & Aims:BRAF-V600E mutation has recently been considered as a molecular marker in diagnosis of Hairy Cell Leukemia (HCL). Detection of this mutation has found a diagnostic and therapeutic value. The aim of the present study was comparing the diagnostic value of BRAF V600E mutation detection with other previous methods in diagnosis of HCL patients. Materials & M...
متن کاملSimple genetic diagnosis of hairy cell leukemia by sensitive detection of the BRAF-V600E mutation.
Hairy cell leukemia (HCL) is a distinct clinicopathologic entity that responds well to purine analogs but is sometimes difficult to differentiate from HCL-like disorders (e.g., splenic marginal zone lymphoma and HCL variant). We recently identified the BRAF-V600E mutation as the disease-defining genetic event in HCL. In this study, we describe a new, simple, and inexpensive test for genetics-ba...
متن کاملImmunohistochemistry for BRAF V600E in the Differential Diagnosis of Hairy Cell Leukemia vs Other Splenic B-Cell Lymphomas.
OBJECTIVES Recent reports have used immunohistochemistry (IHC) with a mutation-specific antibody to detect the BRAF V600E mutation, which is found in nearly all cases of hairy cell leukemia (HCL). To date, however, only a small number of non-HCL, splenic B-cell lymphomas have been examined by IHC. METHODS We analyzed 121 cases, including 26 HCLs, 52 non-HCL splenic lymphomas, 22 chronic lymph...
متن کاملBRAF V600E and MAP2K1 Mutations in Hairy Cell Leukemia and Splenic Marginal Zone Lymphoma Cases
Dear Editor, Differentiation of classic hairy cell leukemia (HCL-c) from HCLvariant (HCL-v) or splenic marginal zone lymphoma (SMZL) is important owing to their different treatment strategies and prognostic implications. Recently, testing for BRAF V600E mutations was suggested as an important diagnostic option for HCL-considering that it was exclusively detected in almost all cases [1]. The BRA...
متن کاملBRAF V600E mutation in hairy cell leukemia: from bench to bedside.
Hairy cell leukemia (HCL) is a distinct clinicopathological entity whose underlying genetic lesion has remained a mystery for over half a century. The BRAF V600E mutation is now recognized as the causal genetic event of HCL because it is somatic, present in the entire tumor clone, detectable in almost all cases at diagnosis (encompassing the whole disease spectrum), and stable at relapse. BRAF ...
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